Abstract

Reactive Oxygen Species (ROS) are involved in the pathogenesis of several diseases, and antioxidants are important for maintaining optimal human health and well-being because of their role in scavenging free radicals. The aim of this study is to evaluate the antioxidant potentials of compound isolated from Tinospora cordifolia. Crude extract of T. cordifolia stem was successively partitioned into nhexane, dichloromethane, ethyl acetate and aqueous fractions. Standard procedures were used to evaluate the (1,1-diphenyl-2-picrylhydrazyl) DPPH radical scavenging activity in vitro. Most active fraction was subjected to chromatographic method to isolate and purify compound. The compound was characterized using spectroscopic analyses (EI-MS, ¹H-NMR, ¹³C-NMR, 2D-NMR, FT-IR, UV). Ethyl acetate fraction had the highest radical scavenging activity with IC50 of 0.419±0.03 mg/mL among tested extracts as compared to standard drug vitamin C (IC50 = 0.008±0.00 mg/mL). The compound Corydine, an isoquinoline alkaloid was identified and was able to scavenge the DPPH radical with IC50 of 0.298±4.38 mM (67.2% inhibition) as compared to N-Acetyl-L-Cysteine (IC50 = 0.115±3.91 mM, 97.5% inhibition) at 0.5 mM concentration. Corydine can possibly mitigate cellular damage caused by reactive oxygen species and help to maintain optimum health and wellbeing.

Keywords: Isoquinoline alkaloid, Corydine, Antioxidant, Tinospora cordifolia, Chromatography